Comparison among nasopharyngeal swab, nasal wash, and oropharyngeal swab for respiratory virus detection in adults with acute pharyngitis
نویسندگان
چکیده
BACKGROUND Acute pharyngitis is frequently seen in primary care. Acute viral pharyngitis may be easily misdiagnosed as acute bacterial pharyngitis. Laboratory-confirmed diagnosis of respiratory viruses is recommended. The purpose of this study was to compare the sensitivities among oropharyngeal swab (OPS), nasopharyngeal swab (NPS), and nasal wash (NW) in adults with acute pharyngitis. METHODS OPS, NPS, and NW were obtained from each participant with acute pharyngitis. The specimens were tested for 15 respiratory viruses by TaqMan real-time polymerase chain reaction. A sample was considered to be a true positive if any of the specimens was positive. The sensitivities among samples were compared by chi-square test or Fisher's exact test, as appropriate. RESULTS One hundred three triple samples collected consecutively by OPS, NPS, and NW were obtained. In 73 patients, one or more viruses were detected by any of the three methods. Among all viruses, the sensitivity of NPS was significantly higher than that of NW (74% vs. 49%, respectively; p < 0.01) and OPS (74% vs. 49%, respectively; p < 0.01). CONCLUSIONS Flocked NPS collection may be the most effective alternative to NW and OPS for detection of respiratory viruses in adults with acute pharyngitis using TaqMan real-time polymerase chain reaction.
منابع مشابه
Comparison among Nasopharyngeal Swab, Nasal Wash and Oropharyngeal Swab for Respiratory Viruses Detection in Adults with Acute Pharyngitis
Background: Acute pharyngitis is one of the most common illnesses for which patients visit primary care physicians. Its etiology is primarily viral in adults. Only about 10% of cases have a bacterial cause. Nonetheless, a total of 78-98% of adults with acute pharyngitis was treated with antibiotics. Thus, virological investigation prior to treating would support the appropriate use of antiviral...
متن کاملIdentification of respiratory viruses in adults: nasopharyngeal versus oropharyngeal sampling.
The optimal method for identifying respiratory viruses in adults has not been established. The objective of the study was to compare the sensitivities of three sampling methods for this purpose. One thousand participants (mean age, 63.1 +/- 17.8 years) were included. Of these, 550 were patients hospitalized for acute febrile lower respiratory tract infections and 450 were controls. Oropharyngea...
متن کاملComparison of nasal and nasopharyngeal swabs for influenza detection in adults.
OBJECTIVE Examine differences in the detection of influenza by specimen and test type using paired nasal and nasopharyngeal swabs. DESIGN Prospective study SETTING Enrollment took place between January and March 2007 in a central Wisconsin population. PARTICIPANTS Adult patients were screened and enrolled by trained research coordinators following medical encounters for acute respiratory ...
متن کاملLaboratory Diagnosis of SARS
The virologic test results of 415 patients with severe acute respiratory syndrome (SARS) were examined. The peak detection rate for SARS-associated coronavirus occurred at week 2 after illness onset for respiratory specimens, at weeks 2 to 3 for stool or rectal swab specimens, and at week 4 for urine specimens. The latest stool sample that was positive by reverse transcription-polymerase chain ...
متن کاملThe acceptability and validity of self‐collected nasal swabs for detection of influenza virus infection among older adults in Thailand
BACKGROUND Self-collection of nasal swabs could improve the timeliness of influenza virus detection in older adults. OBJECTIVES Measure the acceptability, adequacy, timeliness, and validity of self-collected nasal swabs among adults >65 years in Thailand. METHODS Our evaluation consisted of two parts: a one-month study among randomly selected, community-dwelling older adults to simulate com...
متن کامل